LOCUS       ASK2J00166R_GBA1       10415 bp DNA     linear   UNA 25-APR-2024
DEFINITION  glucosylceramidase beta 1 [Source:HGNC Symbol;Acc:HGNC:4177].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      Homo sapiens
  ORGANISM  Homo sapiens
REFERENCE   1  (bases 1 to 10415)
  AUTHORS   Ensembl
  TITLE     Direct Submission
  JOURNAL   Exported Jun 7, 2024 from SnapGene 7.2.0
            https://www.snapgene.com
COMMENT     gene ENSG00000262446
FEATURES             Location/Qualifiers
     source          1..10415
                     /mol_type="genomic DNA"
                     /organism="Homo sapiens"
     gene            1..10415
                     /label=GBA1
                     /note="gene ENSG00000262446
                     Protein coding"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 1..10415
                     /label=GBA1-230
                     /note="primary transcript ENST00000618489"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 193..10411
                     /label=GBA1-224
                     /note="primary transcript ENST00000574670"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 227..6650
                     /label=GBA1-226
                     /note="primary transcript ENST00000575321
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 241..6299
                     /label=GBA1-228
                     /note="primary transcript ENST00000576759
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 257..5228
                     /label=GBA1-216
                     /note="primary transcript ENST00000570491
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 3588..10415
                     /label=GBA1-229
                     /note="primary transcript ENST00000613861"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 3620..4903
                     /label=GBA1-221
                     /note="primary transcript ENST00000573492
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 3629..10411
                     /label=GBA1-220
                     /note="primary transcript ENST00000572338"
                     /note="color: #ffcc00; direction: RIGHT"
     prim_transcript 3632..9847
                     /label=GBA1-227
                     /note="primary transcript ENST00000576421
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             join(3751..3777,4146..4233,4786..4977,5101..5247,6213..6346,
                     6557..6729,7285..7522,8394..8618,9019..9182,9552..9668,
                     9763..9868)
                     /codon_start=1
                     /label=GBA1-220
                     /note="coding sequence ENSP00000459642"
                     /note="This feature has 11 segments:
                         1: 3751 .. 3777 / #993366 / ENSE00002647881
                         2: 4146 .. 4233 / #993366 / ENSE00003503169
                         3: 4786 .. 4977 / #993366 / ENSE00003519054
                         4: 5101 .. 5247 / #993366 / ENSE00003658584
                         5: 6213 .. 6346 / #993366 / ENSE00003585759
                         6: 6557 .. 6729 / #993366 / ENSE00003578412
                         7: 7285 .. 7522 / #993366 / ENSE00003463405
                         8: 8394 .. 8618 / #993366 / ENSE00002631787
                         9: 9019 .. 9182 / #993366 / ENSE00003604870
                        10: 9552 .. 9668 / #993366 / ENSE00003479505
                        11: 9763 .. 9868 / #993366 / ENSE00002683243"
                     /translation="MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIP
                     KSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGL
                     LLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMA
                     SCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPT
                     WLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLS
                     GYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEA
                     AKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRG
                     MQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYH
                     LGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVG
                     FLETISPGYSIHTYLWCRQ"
     CDS             join(3751..3777,4146..4233,4786..4977,5101..5247,6213..6346,
                     6557..6729,7285..7522,8394..8618,9019..9182,9552..9668,
                     9763..9868)
                     /codon_start=1
                     /label=GBA1-224
                     /note="coding sequence ENSP00000458274"
                     /note="This feature has 11 segments:
                         1: 3751 .. 3777 / #993366 / ENSE00003516854
                         2: 4146 .. 4233 / #993366 / ENSE00003503169
                         3: 4786 .. 4977 / #993366 / ENSE00003519054
                         4: 5101 .. 5247 / #993366 / ENSE00003658584
                         5: 6213 .. 6346 / #993366 / ENSE00003585759
                         6: 6557 .. 6729 / #993366 / ENSE00003578412
                         7: 7285 .. 7522 / #993366 / ENSE00003463405
                         8: 8394 .. 8618 / #993366 / ENSE00002631787
                         9: 9019 .. 9182 / #993366 / ENSE00003604870
                        10: 9552 .. 9668 / #993366 / ENSE00003479505
                        11: 9763 .. 9868 / #993366 / ENSE00002683243"
                     /translation="MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIP
                     KSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGL
                     LLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMA
                     SCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPT
                     WLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLS
                     GYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEA
                     AKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRG
                     MQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYH
                     LGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVG
                     FLETISPGYSIHTYLWCRQ"
     CDS             join(3751..3777,4146..4233,4786..4977,6213..6346,6557..6729,
                     7285..7522,8394..8618,9019..9182,9552..9668,9763..9868)
                     /codon_start=1
                     /label=GBA1-229
                     /note="coding sequence ENSP00000482397"
                     /note="This feature has 10 segments:
                         1: 3751 .. 3777 / #993366 / ENSE00002314374
                         2: 4146 .. 4233 / #993366 / ENSE00003503169
                         3: 4786 .. 4977 / #993366 / ENSE00003519054
                         4: 6213 .. 6346 / #993366 / ENSE00003585759
                         5: 6557 .. 6729 / #993366 / ENSE00003578412
                         6: 7285 .. 7522 / #993366 / ENSE00003463405
                         7: 8394 .. 8618 / #993366 / ENSE00002631787
                         8: 9019 .. 9182 / #993366 / ENSE00003604870
                         9: 9552 .. 9668 / #993366 / ENSE00003479505
                        10: 9763 .. 9868 / #993366 / ENSE00002313070"
                     /translation="MEFSSPSREECPKPLSRVSIMAGSLTGLLLLQAVSWASGARPCIP
                     KSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGI
                     GYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVS
                     LLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTA
                     ENEPSAGLLSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHW
                     AKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQ
                     SVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKD
                     TFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDV
                     PLTIKDPAVGFLETISPGYSIHTYLWCRQ"
     prim_transcript 4164..9931
                     /label=GBA1-215
                     /note="primary transcript ENST00000570340
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 4901..8467
                     /label=GBA1-222
                     /note="primary transcript ENST00000573812
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 4901..7522
                     /label=GBA1-223
                     /note="primary transcript ENST00000573998
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             join(4932..4977,5101..5247,6213..6346,6557..6729,7285..7522,
                     8394..8618,9019..9182,9552..9668,9763..9868)
                     /codon_start=1
                     /label=GBA1-230
                     /note="coding sequence ENSP00000482419"
                     /note="This feature has 9 segments:
                        1: 4932 .. 4977 / #993366 / ENSE00003730954
                        2: 5101 .. 5247 / #993366 / ENSE00003658584
                        3: 6213 .. 6346 / #993366 / ENSE00003585759
                        4: 6557 .. 6729 / #993366 / ENSE00003578412
                        5: 7285 .. 7522 / #993366 / ENSE00003463405
                        6: 8394 .. 8618 / #993366 / ENSE00002631787
                        7: 9019 .. 9182 / #993366 / ENSE00003604870
                        8: 9552 .. 9668 / #993366 / ENSE00003479505
                        9: 9763 .. 9868 / #993366 / ENSE00002313070"
                     /translation="MELSMGPIQANHTGTGLLLTLQPEQKFQKVKGFGGAMTDAAALNI
                     LALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEE
                     DTKLKIPLIHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWAR
                     YFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIARDLGPTLAN
                     STHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHR
                     LFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPE
                     GGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAV
                     ALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWCRQ"
     prim_transcript 5972..6931
                     /label=GBA1-217
                     /note="primary transcript ENST00000570868
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     prim_transcript 6154..8681
                     /label=GBA1-218
                     /note="primary transcript ENST00000571863
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     8361..8385
                     /label=PCR Forward
                     /note="color: black; sequence: caaggtccaggatcagttgctcttc; 
                     added: 2024-04-25"
     prim_transcript 8404..9902
                     /label=GBA1-225
                     /note="primary transcript ENST00000574935
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     misc_feature    8421..8520
                     /label=Donor Template SNV -> REV
                     /note="color: #00ccff"
     primer_bind     8421..8520
                     /label=Donor Template SNV -> REV
                     /note="color: black; sequence: 
                     TATGTTCATGGCATTGCTGTACATTGGTACCTGGACTTTCTGGCTCCAGCCAAAGCCAC
                     CCTAGGGGAGACACACCGCCTGTTCCCCAACACCATGCTCT; added: 
                     2024-04-25"
     misc_feature    8480..8482
                     /label=PAM
                     /note="color: #ffff00"
     misc_feature    8483..8502
                     /label=gRNA Protospacer
                     /note="color: #ff0000"
     primer_bind     complement(8483..8502)
                     /label=gRNA Protospacer
                     /note="color: black; sequence: ACAGGCGGTGTGTCTTCCCT; added:
                     2024-04-25"
     misc_feature    8487
                     /label=SNV
                     /note="SNV = A
                     REV = G"
                     /note="color: #ff6600"
     primer_bind     complement(8630..8649)
                     /label=Sanger Sequencing Primer
                     /note="color: black; sequence: ctttgcaggaagggagactg; added:
                     2024-04-25"
     prim_transcript 9028..10410
                     /label=GBA1-219
                     /note="primary transcript ENST00000571897
                     protein_coding_CDS_not_defined"
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(9238..9262)
                     /label=PCR Reverse
                     /note="color: black; sequence: gtggtgtagagtgatgtaagccatc; 
                     added: 2024-04-25"
ORIGIN
        1 ctctctctct ctcgctcgct ctctcgctct ctcgctctct ctcgctcgct ctctcgctct
       61 cgctctctct ctctctccgg ctcgccagcg acacttgttc gttcaacttg accaatgaga
      121 cttgaggaag ggctctgagt cccgcctctg catgagtgac cgtctctttt ccaatccagg
      181 tcccgccccg actccccagg gctgcttttc tcgcggctgc gggtggtcgg gctgcatcct
      241 gccttcagag tcttactgcg cggggcccca gtctccagtc ccgcccaggc gcctttgcag
      301 gctgcggtgg gatttcgttt tgcctccggt tggggctgct gtttctcttc gccgacggta
      361 ggcgtaatga atatttcgac ctttggatct tagctgtccc ctccctgcgt tcgcacttaa
      421 cctttttcac cattattatt attattgtta ttattattat tttttgaggg agtctcgccc
      481 tgtcgcccag gctggagtgt aatggcgcct tcttggctca ctgcaacctc cgcctcccgg
      541 gttcaggcga ttctccgacc tcagcctccc aagtacgtgg gattacaggc acccgccacc
      601 acgcacggct aattttttgt atcttttagt agagacgggg tttcaccatg ttggtcaggc
      661 tggtctccaa ttcctgacct cgtgatccgc ccgcctcggc ctgccaaaca gctgtgatta
      721 taggcgtgag ccaccgcgcc cggccaacca tcattattat ttttaacggt aaggatggtc
      781 agattttact aatgaagaag agattataaa atcttcaagt ctttatatcc acttgctttt
      841 tgaggggtgg agtgggaaga aggttatgta attcatacgt tcttcagaca tgtgacaaac
      901 attcacggag cccggcgacg agcgtcgggg ttgggattcg cactggagct gcagatgggt
      961 gccaggatgg actggtccct accctccgct tgaacctagg aggcggaggt tgcagtgaac
     1021 cgagatcgtg ccactgcact ccagcctggg tgacagagat actccgtctc aaaaaaaaaa
     1081 acaaaacaaa aaacaagcgg actgggcgca gtgcctcacc ctgtaatccc agcactttgc
     1141 aaagccaagg cgggaggatc ctttgagttt aggagtttga gaccaacctg cgcaacacag
     1201 taagaccccg tctctacaaa aaatacagaa attagccagg tgtggtggtg tgcgcctata
     1261 gtcccagcta ttctggaggc tgaggtggga ggattgctta ttctggaggc agaggttgca
     1321 ctgagccgaa atcaagctac tacactccat ccagggcaac atacggagac cctgtctcaa
     1381 acaaacaaac aaaaaattgc tcagtacctg gccaaaaaag aagaggctca ctatgcagag
     1441 gggaagtgga aggagatgtt tggacttcta aactcaatag agcaggagag gcaaatgtag
     1501 aatgtgctca ggaaatatct gtgagatgaa tgaacttgag ggaagtaagg tactagatat
     1561 tacctgccct acccagaaca aatcctgtgc aatgtttcct tgaaaagtga gaagtctgga
     1621 aggggtggct actgacatag tgaagcaact agttcaattc tacaacttga cagctacccc
     1681 tgtgccaggc tatctacgag gatacttaga atgcataaga cattccttca aggaactcca
     1741 ggaacagagg cctgacatgt tgcaatgttt agtgtcaagc agtgtactag agacacatta
     1801 tcacactcaa acctcacaac aattctgtga ggtaggagtt atcactcccc ttttatagat
     1861 gaaacagagg cttagagtga ttgatttatt gaaagtcaaa cagccagtaa atggtgtagc
     1921 caggattcca aacttgctgt ctcactgaga ctgtacttaa ttactggagg gaccgggtgt
     1981 ggtggctcat tgctataatc ccaacacctt gggaggctga ggctggtgga tcacctgagg
     2041 tcaggggttc gagaccagcc tggccaacat ggtgaaaccc catctctact aaaaatacaa
     2101 aaattagctg ggcatggtgg tgggctcctg taatcccagc tactcaggag gctgaggcag
     2161 ggcaattgct tgagccgaga tcacactgca ctccagcctg ggcaacaggg caagactctg
     2221 tctcaaaacc aaaaaaaaaa aaattactgg aggaacctag aagaagaaat gatcaatttt
     2281 gcttggagtg tatctagaaa gacttcactg agatcattta aagaacaaaa aggatggctg
     2341 gggtccagcg cagtggctca tgcctgtaat cccagcactt tcggatacca aggcagcaga
     2401 tcacctgagg tccagagttt cagaccagcc tggccaacat agtgaaaccc catctctact
     2461 aaaaataaaa aaattagctg agcatgttgg agggcacctg taatcccagc tacttgggag
     2521 gctgaggcag gagaatcact cgaacccagg aggtggaggt tgcagtgagc caagatcacg
     2581 ccactgcact ccagcctggg caacagagtg agactctgtc tcaaaaaaca acaacaacaa
     2641 aaaatacaaa caagagacaa gtagttccca ggtgcctacc aagtggtcag gcactgcact
     2701 tacctcactg actgcagtaa ccaccctttg aggttgtggc attgcctcca ttttccaggc
     2761 aaggaaatgg gctgagagct gggattagtc aggtcatgac tgtgtgtgcc actcccgcta
     2821 aatctcattt gatgtggttc atgaggccac accatggaca gcttcctcct tgtgtccact
     2881 gaggatatgg ctttgtacaa cactttggtt tttgaacgac tttacaaacc tccctgtctt
     2941 gtgaggaagg aagaacagtt attaccatct gcatctgatg atgaaacaag ggacgctgca
     3001 gaggagccgc actgaccact ccctccctcc agtcctgtca tcccactgcc agtgtcccac
     3061 cctcttgtgc cctgcacttc actggctaat aacccccctc actttttcct ctgtgaagcc
     3121 atcctggata attccccacc cacgaatggt ccctcctcat ctcagagagc tctccatgca
     3181 cacctgttac cgtttctgtc tttatctgta aatatctgtg tgtctgactt ccatgcctca
     3241 cacacctcta tagggcaaag actgtcttaa acatcttggt agtgtcagta ttttgcacag
     3301 tgaagttttt ttttttaaat tatatcagct ttatttgtac ctttttgaca tttctatcaa
     3361 aaaagaagtg tgcctgctgt ggttcccatc ctctgggatt taggagcctc taccccattc
     3421 tccatgcaaa tctgtgttct aggctcttcc taaagttgtc acccatacat gccctccaga
     3481 gttttatagg gcatataatc tgtaacagat gagaggaagc caattgccct ttagaaatat
     3541 ggctgtgatt gcctcacttc ctgtgtcatg tgacgctcct agtcatcaca tgacccatcc
     3601 acatcgggaa gccggaatta cttgcagggc taacctagtg cctatagcta aggcaggtac
     3661 ctgcatcctt gtttttgttt agtggatcct ctatccttca gagactctgg aacccctgtg
     3721 gtcttctctt catctaatga ccctgagggg atggagtttt caagtccttc cagagaggta
     3781 agagagagag ctcccaatca gcattgtcac agtgcttctg gaatcctggc actggaattt
     3841 aatgaatgac agactctctt tgaatccagg gccatcatgg ctctttgagc aaggcacaga
     3901 tggagggagg ggtcgaagtt gaaatgggtg ggaagagtgg tggggagcat cctgatttgg
     3961 ggtgggcaga gagttgtcat cagaagggtt gcagggagag ctgcacccag gtttctgtgg
     4021 gccttgtcct aatgaatgtg ggagaccggg ccatgggcac ccaaaggcag ctaagccctg
     4081 cccaggagag tagttgaggg gtggagaggg gcttgctttt cagtcattcc tcattctgtc
     4141 ctcaggaatg tcccaagcct ttgagtaggg taagcatcat ggctggcagc ctcacaggat
     4201 tgcttctact tcaggcagtg tcgtgggcat caggtgagtg agtcaaggca gtggggaggt
     4261 agcacagagc ctcccttctg cctcatagtc ctttggtagc cttccagtaa gctggtggta
     4321 gacttttagt aggtgctcaa taaatccttt tgagtgactg agaccaactt tggggtgagg
     4381 attttgtttt ttttcttttg aaacagagtc ttactctgtt gcctgggctg gagtgcagtg
     4441 gtgcaatttt ggctcattcc aacctctgcc tcccagattc aagcgattct cttgcttcag
     4501 cttcccaggt agctgggatt acaggcggcc accactacgc ccagctaatt tttgtatttt
     4561 tagtagagac ggggtttcac catgctggca aggcaggtct caaactcctc acctcaggtg
     4621 atccgcccac ctcggcctcc taaagtgcta ggattacagg tgtgagcccc tgcgcccggc
     4681 caaggggtga ggaattttga aaccgtgttc agtctctcct agcagatgtg tccattctcc
     4741 atgtcttcat cagacctcac tctgcttgta ctccctccct cccaggtgcc cgcccctgca
     4801 tccctaaaag cttcggctac agctcggtgg tgtgtgtctg caatgccaca tactgtgact
     4861 cctttgaccc cccgaccttt cctgcccttg gtaccttcag ccgctatgag agtacacgca
     4921 gtgggcgacg gatggagctg agtatggggc ccatccaggc taatcacacg ggcacaggta
     4981 accattacac ccctcacccc ctgggccagg ctgggtcctc ctagaggtaa atggtgtcag
     5041 tgatcaccat ggagtttccc gctgggtact gataccctta ttccctgtgg atgtcctcag
     5101 gcctgctact gaccctgcag ccagaacaga agttccagaa agtgaaggga tttggagggg
     5161 ccatgacaga tgctgctgct ctcaacatcc ttgccctgtc accccctgcc caaaatttgc
     5221 tacttaaatc gtacttctct gaagaaggtg aggaggaagg ggacaagatg acatagagcc
     5281 attgaaactt ttcgtttttc ttttcttttt ttaaaatttt tttgaggcag aatctcactc
     5341 tgcccattct gtcggcgaga caggagtgca gtggtgtgat ctcccctcac agcaacctct
     5401 gcctcccagg ctatagtgat tctcctgcct cagcctcctg agtagctgga attataggcg
     5461 tgcgccacta ccacctggct aatttttgta tttttagtag agacagggtt tcatcatgtt
     5521 gaccaggcta gtcttaaact cctgacctca aatgatatac ctgccttggc ctcccgaagt
     5581 gctggaatta caagtgtgag ccaccgagcc cagcagacac ttttcttttt tctttttttt
     5641 tttttgagac agagtctcgc actgtcaccc aggctggagt gcagtggcac aatctcagct
     5701 cactgcaacc tccacctccc gggttcaggt gattctcctg tctcagcctc tcgagtacct
     5761 gggattacag gtgcctgcca ccacgcccgg ctaatttttt gtatttttag tagagacagg
     5821 gtttcactat gttggccagg atgattgcga actcctgacc tcgtgatctg cccacatcgg
     5881 cctcccaaag tgctgggatt acatgcgtga gccactgaca cttttctttg ccctttcttt
     5941 ggaccctgac ttctgcccat ccctgacatt tggttcctgt tttaatgccc tgtgaaataa
     6001 gatttcaccg cctatcatct gctaactgct acggactcag gctcagaaag gcctgcgctt
     6061 cacccaggtg ccagcctcca caggttccaa cccaggagcc caagttccct ttggccctga
     6121 ctcagacact attaggactg gcaagtgata agcagagtcc catactctcc tattgactcg
     6181 gactaccata tcttgatcat ccttttctgt aggaatcgga tataacatca tccgggtacc
     6241 catggccagc tgtgacttct ccatccgcac ctacacctat gcagacaccc ctgatgattt
     6301 ccagttgcac aacttcagcc tcccagagga agataccaag ctcaaggtag gcattctagc
     6361 tttttcaggc cctgagggcc ctgatgtctg ggggttgaga aactgtaggg taggtctgct
     6421 tgtacagaca ttttgtcccc tgctgttttg tcctgggggt gggagggtgg aggctaatgg
     6481 ctgaaccgga tgcactggtt gggctagtat gtgttccaac tctgggtgct tctctcttca
     6541 ctacctttgt ctctagatac ccctgattca ccgagccctg cagttggccc agcgtcccgt
     6601 ttcactcctt gccagcccct ggacatcacc cacttggctc aagaccaatg gagcggtgaa
     6661 tgggaagggg tcactcaagg gacagcccgg agacatctac caccagacct gggccagata
     6721 ctttgtgaag taagggatca gcaaggatgt gggatcagga ctggcctccc atttagccat
     6781 gctgatctgt gtcccaaccc tcaacctagt tccacttcca gatctgcctg tcctcagctc
     6841 acctttctac cttctgggcc tttcagcctt gggcctgtca atcttgccca ctccatcagg
     6901 cttcctgttc tctcggtctg gcccactttc tttttatttt tcttcttttt tttttttttg
     6961 agaaggagtc tctctctctg tcacccaggc tggagtgctg tggcgccatc ttcactcact
     7021 gtaacctctg cctcctgagt tcaagcaatt ctcctgcctc agccttccaa gtagctggga
     7081 ttataggcgc ctgccaccag gcccagctga tttttctatt tttagtagag acggggtttc
     7141 gccaggctgt tctcgaactc ctgaactcaa gtgatccacc tgcctcggct tcccaaagtg
     7201 ctgggattac aggtgtgagc caccacaccc agctggtctg gtccactttc ttggccggat
     7261 cattcatgac ctttctcttg ccaggttcct ggatgcctat gctgagcaca agttacagtt
     7321 ctgggcagtg acagctgaaa atgagccttc tgctgggctg ttgagtggat accccttcca
     7381 gtgcctgggc ttcacccctg aacatcagcg agacttcatt gcccgtgacc taggtcctac
     7441 cctcgccaac agtactcacc acaatgtccg cctactcatg ctggatgacc aacgcttgct
     7501 gctgccccac tgggcaaagg tggtaaggcc tggacctcca tggtgctcca gtgaccttca
     7561 aatccagcat ccaaatgact ggctcccaaa cttagagcga tttctctacc caactatgga
     7621 ttcctagagc accattcccc tggacctcca gggtgccatg gatcccacag ttgtcgcttg
     7681 aaacctttct aggggctggg cgaggtggct cactcatgca aacccagcac tttgggaagc
     7741 cgaggcgggt gatcacctga ggtcaggagt ttaagaccac cctggccaac gtgttgaaac
     7801 cctgtgtcta ctaaaataca aaaaaaaaaa attatctggg catgatggtg ggtgtctgta
     7861 atcccagcta ctcaggaggc tgagaaggga gaatcagttg aacccgggag atggtggttg
     7921 cggtgagccg agatcgcgcc actgcactcc agcctgggag gctgagcgag actccatctc
     7981 gaaacaaaac aaaacaaaac tatctaggct gggggtggtg gttcatgtat gtatgtgtat
     8041 atacatatat atgtgtttat atgtatatat atatacacac acacacatac atacacacac
     8101 atacacacac aaattagctg ggtgtggcac ccgtgtagtc ccagctactc aggaggctaa
     8161 tgtgggagga tcagttgacc ctaggaagtc aaggctgcag tgagtcgtga ttgcgccact
     8221 gtactccagc ccgagtgaca gagtgacatc ctgtctcaaa aacaaaaaaa aatctcccca
     8281 aacctctcta gttgcattct tcccgtcacc caactccagg attcctacaa caggaactag
     8341 aagttccaga agcctgtgtg caaggtccag gatcagttgc tcttcctttg caggtactga
     8401 cagacccaga agcagctaaa tatgttcatg gcattgctgt acattggtac ctggactttc
     8461 tggctccagc caaagccacc ctaggggaga cacaccgcct gttccccaac accatgctct
     8521 ttgcctcaga ggcctgtgtg ggctccaagt tctgggagca gagtgtgcgg ctaggctcct
     8581 gggatcgagg gatgcagtac agccacagca tcatcacggt aagccacccc agtctccctt
     8641 cctgcaaagc agacctcaga cctcttacta gtttcaccaa agactgacag aagcccttcc
     8701 tgtccagctt tccccagcta gcctgccctt ttgagcaact ctggggaacc atgattccct
     8761 atcttccctt tccttcacag gtctgcacac ctcattgccc cttttgcaac tactgaggca
     8821 cttgcagctg cctcagactt ctcagctccc cttgagatgc ctggatcttc acacccccaa
     8881 ctccttagct actaaggaat gtgcccctca cagggctgac ctacccacag ctgcctctcc
     8941 cacatgtgac ccttacctac actctctggg gacccccagt gttgcgcctt tgtctctttg
     9001 cctttgtcct taccctagaa cctcctgtac catgtggtcg gctggaccga ctggaacctt
     9061 gccctgaacc ccgaaggagg acccaattgg gtgcgtaact ttgtcgacag tcccatcatt
     9121 gtagacatca ccaaggacac gttttacaaa cagcccatgt tctaccacct tggccacttc
     9181 aggtgagtgg agggcgggca cccccattcc ataccaggcc tatcatctcc tacatcggat
     9241 ggcttacatc actctacacc acgagggagc aggaaggtgt tcagggtgga acctcggaag
     9301 aggcacaccc atcccctttt gcaccatgga ggcaggaagt gactaggtag caacagaaaa
     9361 ccccaatgcc tgaggctgga ctgcgatgca gaaaagcagg gtcagtgccc agcagcatgg
     9421 ctccaggcct agagagccag ggcagagcct ctgcaggagt tatggggtgg gtccgtgggt
     9481 gggtgacttc ttagatgagg gtttcatggg aggtaccccg agggactctg accatctgtt
     9541 cccacattca gcaagttcat tcctgagggc tcccagagag tggggctggt tgccagtcag
     9601 aagaacgacc tggacgcagt ggcactgatg catcccgatg gctctgctgt tgtggtcgtg
     9661 ctaaaccggt gagggcaatg gtgaggtctg ggaagtgggc tgaagacagc gttgggggcc
     9721 ttggcaggat cacactctca gcttctcctc cctgctccct agctcctcta aggatgtgcc
     9781 tcttaccatc aaggatcctg ctgtgggctt cctggagaca atctcacctg gctactccat
     9841 tcacacctac ctgtggtgtc gccagtgatg gagcagatac tcaaggaggc actgggctca
     9901 gcctgggcat taaagggaca gagtcagctc acacgctgtc tgtgactaaa gagggcacag
     9961 cagggccagt gtgagcttac agcgacgtaa gcccaggggc aatggtttgg gtgactcact
    10021 ttcccctcta ggtggtgcca ggggctggag gcccctagaa aaagatcagt aagccccagt
    10081 gtccccccag cccccatgct tatgtgaaca tgcgctgtgt gctgcttgct ttggaaactg
    10141 ggcctgggtc caggcctagg gtgagctcac tgtccgtaca aacacaagat cagggctgag
    10201 ggtaaggaaa agaagagact aggaaagctg ggcccaaaac tggagactgt ttgtctttcc
    10261 tggagatgca gaactgggcc cgtggagcag cagtgtcagc atcagggcgg aagccttaaa
    10321 gcagcagcgg gtgtgcccag gcacccagat gattcctatg gcaccagcca ggaaaaatgg
    10381 cagctcttaa aggagaaaat gtttgagccc agtca
//
